Agarwal and Kaur: To study the changes in platelets both qualitative and quantitative in all pregnant and compare with nonpregnant women of same age group


Pregnancy is related with typical physiological changes that help the sustaining and endurance of the embryo. Biochemical boundaries mirror these versatile changes in most organ framework and are unmistakably particular from the non-pregnant state.1 However, these progressions become vital in case of difficulties. Pregnancy entanglements may incorporate sickliness, hyperlipidemia, hypertension, diabetes and preeclampsia.2 Each year, as per the WHO, medical affliction because of pregnancy is capable (once in a while forever) by in excess of 20 million ladies around the globe. Moreover, the "existences of 8,000,000 ladies are compromised, and in excess of 500,000 ladies are assessed to have kicked the bucket because of makes related pregnancy and childbirth.3

Fruitful result of pregnancy requires regular checking of biochemical and hematological boundaries to stay away from complexities all through the trimesters of pregnancy. A few creators have been accounted for aggravations in sugar metabolism,4 lipid profile,5 protein profile,6 liver capacity and kidney function,14 during pregnancy. Hematological changes were additionally documented.7

Iron deficiency is the most well-known hematological issue in pregnancy, trailed by thrombocytopenia. The Leukocytosis is quite often connected with pregnancy. 8

Materials and Methods

400 consecutive blood samples of pregnant women’s as study group and 400 consecutive blood samples of non-pregnant women’s as control group, were included in the study. Each blood sample was mixed well and then approximately 20 μL was aspirated by allowing the analyzer’s sampling probe into the blood sample and depressing the start button. Results of the analysis were displayed after about 30 seconds, after which the analyzer generated a paper copy of the results on thermal printing paper.

Inclusion criteria

  1. Women’s of 20 to 40 years attending antenatal clinic.

  2. Women’s giving urine pregnancy test positive or are positive for live fetus in ultrasonography was included in our study as study group and who are negative for the same was taken as control group.

Exclusion criteria

Patient with

  1. Bleeding disorders

  2. Splenomegaly

  3. Connective tissue disease such as systemic lupus erythematosus

  4. Hypertension

  5. Human immunodeficiency virus (HIV)

  6. Hepatitis B infection

  7. Women on non-steroidal anti-inflammatory drugs such as aspirin were also excluded


Table 1

Comparison of Mean Platelet between the groups (N=400)


First Trimester (n=156)

Second Trimester (n=155)

Third Trimester (n=89)

(Mean ± SD)

(Mean ± SD)

(Mean ± SD)


2.02 ± 0.69

1.92 ± 0.70

2.05 ± 0.69

The F value obtained was 1.27 with a P value of > 0.05, which is statistically not significant. Thus, platelet values in all the three groups is comparable. As the ANOVA value was found to be non-significant, Post-hoc Tukey test has not been applied.

Table 2

Comparison of Mean Lymphocytes between the groups (N=400)


First Trimester (n=156)

Second Trimester (n=155)

Third Trimester (n=89)

(Mean ± SD)

(Mean ± SD)

(Mean ± SD)


23.68 ± 6.35

19.47 ± 6.81

21.75 ± 7.66

Significant difference was seen between the pairs first trimester - second trimester; and second trimester - third trimester, while non-significant difference was seen between first trimester – third trimester pair. One-Way ANOVA test was used. F value = 14.79, P value = 0.000, Significant

The F value obtained was 14.79 with a P value of < 0.05, which is statistically significant. Thus, lymphocyte values in all the three groups are statistically different.

Table 3

Comparison of Mean Monocytes between the groups (N=400)


First Trimester (n=156)

Second Trimester (n=155)

Third Trimester (n=89)

(Mean ± SD)

(Mean ± SD)

(Mean ± SD)


3.56 ± 1.29

3.20 ± 1.28

3.49 ± 1.65

One-Way ANOVA test was used. F value = 2.96, P value = 0.053, Not Significant. The F value obtained was 2.96 with a P value of > 0.05, which is statistically not significant. Thus, onocytes values in all the three groups is comparable. As the ANOVA value was found to be non-significant, Post-hoc Tukey test has not been applied.


Red platelet check, hemoglobin substance and hematocrit esteem were fundamentally lower in pregnant ladies contrasted with non-pregnant ladies. Mean corpuscular volume and MCH were not extraordinary while MCHC showed progressive huge decline in pregnant ladies. Comparable outcomes were reported (James et al., 2008 and Chandra et al., 2012).9 During pregnancy, an expanded plasma volume with the absence of a satisfactory expansion in erythrocytes mass outcomes in a reduction in hemoglobin level and the advancement of paleness, which is characterized as weakening frailty.10 Furthermore the overall lessening in various blood records is almost certain clarified by expanded necessities during pregnancy. In this manner, the increment in these blood files is an impression of sufficient iron stock bringing about expanded hemoglobin creation. Notwithstanding, the part of nourishment and additionally iron supplementation was not assessed (Papadopol et al., 2001).11

Platelet include was continuously diminished in pregnant ladies contrasted with non-pregnant ladies as the pregnancy progressed. Critical reductions in platelet check of pregnant ladies contrasted with non-pregnant ladies (P value<0.05) in concurrence with study announced that: despite the fact that platelet includes stay in the ordinary pregnant reach in most ladies during simple pregnancies mean platelet tallies of pregnant ladies might be marginally lower than in solid non pregnant women.12

The most successive hematologic complexity during pregnancy is paleness. Various ordinary physiologic cycles happen during pregnancy prompting the expression "physiologic iron deficiency of pregnancy". The plasma volume expands (40–half) comparative with red cell mass (20–30%) and represents the fall in hemoglobin focus. Be that as it may, if the hemoglobin falls under 11 gm/dL an assessment for iron inadequacy paleness (IDA) ought to be started since iron lack is liable for most of anemias analyzed during pregnancy. Frailty auxiliary to press inadequacy is the most continuous hematologic difficulty and is effectively treated with oral iron plans, anyway care should be taken not to miss different reasons for paleness, for example, sickle cell disease.13

Garn and et al morbidogenic nature of low hemoglobin and haematocrit levels during pregnancy and furthermore showed the disavantages of high maternal hemoglobin and haematocrit regarding pregnancy outcome.14 Additionally Yip showed that moderate paleness is frequently connected with poor perinatal results.

One should take care to try not to accomplish high iron stores since certain reports recommend prophylactic supplementation might be destructive to pregnant ladies that are not iron inadequate. Weakness adds to intrauterine development limitation, preterm work, early terminations and it is likewise an essential driver of low insusceptibility of both the mother and the infant, which makes them inclined for a few hazardous diseases. Hemoglobin assessment is the main boundary to begin supplement of iron or eliminating different reasons for frailty to forestall horrible pregnancy outcomes.15


The estimation of anemia prevalence is an important step for health policy makers, as hemoglobin estimation is most important parameter to start supplementation of iron or removing other cause of anemia to prevent unfavorable outcome. 5. In order to get better pregnancy care more accurate and reliable figures, uniform and standard methods and study designs are also recommended for primary studies at the national level.

Source of Funding

No financial support was received for the work within this manuscript.

Conflict of Interest

The authors declare they have no conflict of interest.



M Goonewardene M Shehata Anemia in pregnancy Best Pract Res Clin Obstet Gynecol2011261324


F O Okoji O Idonije E Blessing A Mabel O Okhiai U Faith Comparative study of lipid profile of normal pregnant women in the different trimesterArch Appl Sci Res20113352832


World Health Organization. Reproductive Health and Research. Regional Office for South-East Asia2009


A I Salisu M K Atiku Serum lipid profile in non- pregnant and pregnant hausa-fulani women at second and third trimesters of pregnancy in Kura local govenment areaBayero J Pure Appl Sci2009221313


D Mankuta M Elami-Suzin A Elhayani S Vinker RLeispeaircdh profile in consecutive pregnanciesLipids Health Dis2010


EJA Buul EAP Steegers H W Jongsma TKAB Eskes CMG Thomas P R Hein Haematological and biochemical profile of uncomplicated pregnancy in nulliparous womenNeth J Med1995467385


M Imoru A Emeribe Changes in plasma proteins and fibrinolytic activity in pregnant women in calabarInt J Gynecol Obstet2010122


F Rodríguez-Dennen J Martínez-Ocaña S Kawa-Karasik L Villanueva-Egan N Reyes-Paredes A Flisser Comparison of hemodynamic, biochemical and hematological parameters of healthy pregnant women in the third trimester of pregnancy and the active labor phaseBMC Pregnancy Childbirth201111110.1186/1471-2393-11-33


T R James H L Reid A M Mullings Are published standards for haematological indices in pregnancy applicable across populations: an evaluation in healthy pregnant Jamaican womenBMC Pregnancy Childbirth20088110.1186/1471-2393-8-8


M Getachew D Yewhalaw K Tafess Yehenew Getachew andAhmed Zeynudin. Anaemia and associated risk factors among pregnant women in Gilgel Gibe dam areaParasit Vectors20125296


H S Lee M S Kim M H Kim Y J Kim W Y Kim Iron status and its association with pregnancy outcome in Korean pregnant womenEur J Clin Nutr20066091130510.1038/sj.ejcn.1602429


S Das D Char S Sarkar T K Saha S Biswas Tushar Kanti Saha, Dr. Sucheta Biswas. Study of Hematological Parameters in PregnancyIOSR J Dent Med Sci 2013121424


Patrick Chukwuyenum Ichipi-Ifukor Juliet Jacobs Rita Ngozi Ichipi-Ifukor, Oberhiri Lawrence Ewrhe. Changes in Haematological Indices in Normal PregnancyPhysiology Journal2013


S M Garn M T Keating F Falkner Hematological status and pregnancy outcomesAm J Clin Nutr1981341115710.1093/ajcn/34.1.115


R Yip Significance of an abnormally low or high hemoglobin concentration during pregnancy: Special consideration of iron nutritionAm J Clin Nutr20007212729


© 2021 Published by Innovative Publication Creative Commons Attribution 4.0 International License (

  • Article highlights
  • Article tables
  • Article images

View Article

PDF File   Full Text Article


PDF File   XML File   ePub File

Digital Object Identifier (DOI)

Article DOI

Article Metrics

Article Access statistics

Viewed: 111

PDF Downloaded: 38