Storage artefacts in peripheral blood smears

Original Article

Author Details : Shaista Choudhary, Ravi Shankar Katkar, Divya Nagaram

Volume : 3, Issue : 3, Year : 2018

Article Page : 187-191

Suggest article by email


Introduction: Microscopic evaluation of peripheral blood smear is a very important investigation. Blood for various laboratory analyses are commonly kept in ethylene diamine tetra acetic acid (EDTA). EDTA can however cause morphological and fragility changes in blood cells. However, morphological analyses can be greatly hampered due to occurrence of artefacts.
Aims and Objectives: To identify the storage artefacts and thus avoid misinterpretation of peripheral blood smears.
Materials and Methods: 50 blood samples were obtained using EDTA and 3.5% sodium citrate as anticoagulants. Smears were made immediately as well as after 2, 4 and 6hrs of storage at room temperature. Smears obtained from same patients by finger prick method served as controls.
Conclusion: EDTA has been recommended as the anticoagulant of choice for peripheral smear but should be examined within 1hr of collection to avoid misinterpretation as pathological findings leading to wrong diagnosis.

Keywords: Peripheral smears, Anticoagulant, Platelets, Neutrophils, Artifacts.

How to cite : Choudhary S, Katkar R S, Nagaram D, Storage artefacts in peripheral blood smears. IP J Diagn Pathol Oncol 2018;3(3):187-191

Copyright © 2018 by author(s) and IP J Diagn Pathol Oncol. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License (

View Article

PDF File  


PDF File    

Digital Object Identifier (DOI)

Article DOI

Article Metrics

Article Access statistics

Viewed: 946

PDF Downloaded: 440